Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 (DE3) cells | Cutaneotrichosporon cutaneum |
expressed in Escherichia coli JM109 (DE3) cells | Fusarium oxysporum |
Protein Variants | Comment | Organism |
---|---|---|
G76A | the mutation decreases the NADH-dependent activity to 40% of the wild type activity | Fusarium oxysporum |
K77A | the mutation decreases the NADH-dependent activity | Fusarium oxysporum |
Q78A | the mutation slightly improves the NADH-dependent activity | Fusarium oxysporum |
S73A | the mutation decreases the NADH-dependent activity | Fusarium oxysporum |
S73G/S75G | the double mutation significantly improves NADPH-dependent Nor activity without affecting, or even slightly increasing, the NADHd-ependent activity | Fusarium oxysporum |
S75A | the mutation decreases the NADH-dependent activity | Fusarium oxysporum |
S75G | the mutation significantly improves the NADPH-dependent Nor activity of isozyme Fnor, and the overall activity is accelerated via the NADPH-enhanced reduction step | Fusarium oxysporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutaneotrichosporon cutaneum | - |
- |
- |
Fusarium oxysporum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography and Mono Q or Resource Q column chromatography | Cutaneotrichosporon cutaneum |
DEAE-cellulose column chromatography and Mono Q or Resource Q column chromatography | Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NO + NADH + H+ | isozyme Fnor utilizes only NADH as electron donor | Fusarium oxysporum | N2O + NAD+ + H2O | - |
? | |
NO + NADH + H+ | isozyme Tnor utilizes both NADH and NADPH as electron donors | Cutaneotrichosporon cutaneum | N2O + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450nor | - |
Cutaneotrichosporon cutaneum |
cytochrome P450nor | - |
Fusarium oxysporum |
Fnor | isozyme | Fusarium oxysporum |
NOR | - |
Cutaneotrichosporon cutaneum |
NOR | - |
Fusarium oxysporum |
P450nor | - |
Cutaneotrichosporon cutaneum |
P450nor | - |
Fusarium oxysporum |
Tnor | isozyme | Cutaneotrichosporon cutaneum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Cutaneotrichosporon cutaneum | |
heme | - |
Fusarium oxysporum | |
NADH | isozyme Fnor utilizes only NADH as electron donor | Fusarium oxysporum | |
NADH | isozyme Tnor utilizes both NADH and NADPH as electron donors | Cutaneotrichosporon cutaneum | |
NADPH | isozyme Tnor utilizes both NADH and NADPH as electron donors | Cutaneotrichosporon cutaneum |